Unexpected mutations from CRISPR/Cas9 editing in-vivo

Nat Methods. 2017 May 30;14(6):547-548. doi: 10.1038/nmeth.4293.
Schaefer KA, Wu WH, Colgan DF, Tsang SH, Bassuk AG, Mahajan VB
CRISPR–Cas9 editing shows promise for correcting disease-causing mutations. For example, in a recent study we used CRISPR-Cas9 for sight restoration in blind rd1 mice by correcting a mutation in the Pde6b gene1. However, concerns persist regarding secondary mutations in regions not targeted by the single guide RNA (sgRNA).
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